Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Mucosa-associated bacteria isolated from child intestinal biopsies using the Ham's F-12 enrichment-filtration method.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Bacteria, Isolation, Infection

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Comparison of the percentage invasion and adherence into Caco-2 cells of eight Campylobacter concisus strains.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Comparison

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: C. concisus UNSW2 was observed as spiral curved-shaped bacteria with rounded ends and a single polar flagellum as shown in Panel A (bar = 3 µm). In Panel B (bar = 1.5 µm) C. concisus UNSW3 was observed to be curved-shaped bacteria with rounded ends and a single polar flagellum, while in Panels C (bar = 2 µm) and D (bar = 2.5 µm) C. concisus strains UNSW1 and UNSWCD were shown to be spiral curved-shaped bacterium with rounded ends and a single flagellum.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Bacteria

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Panel A shows an overview of uninfected Caco-2 monolayer. The Caco-2 cells expressed differentiating microvilli (Panel A1) and differentiated microvilli (Panel A2). C. concisus was shown to aggregate upon interaction with host cells as shown in Panel B (Panel B1, bar = 1.5 µm and Panel B2, bar = 2 µm). In Panel C, the polar flagellum of C. concisus is shown binding to the tips of host cell microvilli which mediated initial contact with host cells (as indicated by the arrows). Abnormalities in the epithelial host cell structure and microvilli were observed following infection with C. concisus (indicated by a ring in Panel D and arrows in Panel E). Panel F shows the flagellum of C. concisus appeared to wrap itself around the microvilli (as indicated by arrows). Following adherence, C. concisus induced a “membrane ruffling”-like effect on the host cell membrane (indicated by an asterisk in Panel F), and penetrated the host cell membrane from the non-flagellated end (indicated by an arrow in Panel G). C. concisus was observed invading the host cell (indicated by arrows in Panels G, H and I) resulting in irregular shaped membrane protrusions (indicated by asterisks in Panels G, H and I), leading to host cell damage (indicated by “#” in Panels G, H and I).

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Binding Assay, Infection, Membrane

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Panel A shows uninfected LS174T monolayers. LS174T cells expressing microvilli (indicated by a ring) and goblet cells (indicated by a arrow) are shown in Panel B. The mucus layer was found on the monolayer surface of LS174T cells as indicated by an “#” in Panel C. C. concisus appeared to be attracted to the mucus layer of host cells (indicated by “#” in Panel D) using their single polar flagellum (indicated by arrows in Panel E) and upon the interaction with host cells tended to aggregate (Panel F). Panel G shows the polar flagellum (as indicated by an arrow) of C. concisus binding to the tips of host cell microvilli (as indicated by a ring) and goblet cells (as indicated by an arrow in Panel H) which appeared to mediate initial contact with host cells. Following adherence, C. concisus induced a “membrane ruffling”-like effect on the host cell membrane (indicated by an asterisk in Panel I) and penetrated the host cell membrane from the non-flagellated end (indicated by an arrow in Panel I) resulting in cell damage (indicated by “#” in Panel I).

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Expressing, Binding Assay, Membrane

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Lane 1: FN-1 marker, lane 2: UNSWCD, lane 3: UNSW2, lane 4: UNSW3, lane 5: UNSW1, lane 6: BAA-1457, lane 7: UNSWCS, lane 8: ATCC 51562, lane 9: ATCC 51651 and lane 10: negative control.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Marker, Negative Control

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Caco-2 cell proteins whose expression is upregulated in the presence of Campylobacter concisus UNSWCD.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Expressing, Clinical Proteomics, Membrane, Binding Assay, RNA Binding Assay, Ubiquitin Proteomics

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Caco-2 cell proteins whose expression is downregulated in the presence of Campylobacter concisus UNSWCD.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Expressing, Clinical Proteomics, Membrane, Binding Assay, RNA Binding Assay, Ubiquitin Proteomics

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: (A) Non-invasive C. concisus strains adhere to the host cell and induce the production of IL-12. (B) Invasive C. concisus strains adhere to and invade the host cell inducing both IL-12 and IFN-γ, which in turn activate the immunoproteosome. The bacterial insult upregulates ubiquitinating and downregulates de-ubiquitinating enzymes which leads to the ubiquitination of NF-κB inhibitors. The immunoproteosome targets these inhibitors which activates NF-κB.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Ubiquitin Proteomics